Using Your Column
Installation
• The direction of flow is marked on the column.
• 1.8 µm columns (ZORBAX RRHT, ZORBAX RRHD) can only be operated in the
direction flow marked on the column.
• Agilent recommends InfinityLab Quick Connect fittings (5067-5961 for 0.075 x
105 mm) or Quick Turn fittings (5067-5966) for a perfect column connection,
every time. Polyketone fittings (5042-8957) can also be used for columns up to
600 bar
Column conditioning
Every column is tested before shipment and shipped in the test eluent. So, for first
use, rinsing with water is not necessary. If mobile phase additives are used (such as
buffers or ion-pair reagents) it is advisable to do an intermediate flush with a mobile
phase of the correct composition, but without these additions. Flushing with 10 to 20
column volumes should help in transitioning to your mobile phase. For shorter chain
chemistries (C8, Phenyl, CN, for example), care should be taken to make sure the
column has been properly equilibrated prior to use. This will ensure reproducibility
and help prevent retention time drifting.
Important safety considerations
• All points of connection in liquid chromatographic systems are potential sources
of leaks. Users should be aware of the toxicity or flammability of their mobile
phases.
• Because of the small particle size, dry column packings are respirable. Columns
should only be opened in a well-ventilated area.
• Please adhere to operating pressure limits noted for each column (see chart).
Exceeding these limits will compromise chromatographic performance and could
be unsafe.
ENGLISH
Search Agilent.com for “quick connect fitting” to learn more
Polyketone fitting,
p/n 5042-8957
Agilent 1200 bar removable
fitting, p/n 5067-4733
Agilent Quick Connect fitting,
p/n 5067-5961
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