Column Operating Parameters: pH and Temperature
Phase Recommended
pH range
Maximum operating
temperature
InfinityLab Poroshell 120 EC-C18,
EC-C8 and Bonus-RP.
ZORBAX Eclipse Plus C18 and C8.
ZORBAX Eclipse XDB-C18,
XDB-C8 and XDB-Phenyl ZORBAX
Bonus-RP.
HC-C18(2) and TC-C18(2).
pH 2.0 to 9.0 60 °C
InfinityLab Poroshell 120 PFP and
Phenyl-Hexyl.
ZORBAX Eclipse Plus Phenyl-Hexyl.
ZORBAX XDB-CN.
ZORBAX Eclipse PAH.
ZORBAX ODS and Phenyl.
All Pursuit, Polaris, Microsorb,
Metasil, Monochrom, Hypersil,
OmniSpher, MicroSpher bonded
phases.
pH 2.0 to 8.0 60 °C
ZORBAX Eclipse PAH pH 2.0 to 8.0 60 °C at pH <6.0
ZORBAX TMS pH 2.0 to 7.0 60 °C
InfinityLab Poroshell 120 SB-C18,
SB-C8 and SB-Aq.
ZORBAX StableBond SB-C18, SB-
C8, SB-Aq, SB-C3 and SB-Phenyl
ZORBAX Rx-C8.
pH 1.0 to 8.0 90 °C (StableBond C18
and Poroshell 120 SB-C18)
80 °C (Rx-C8 and SB-Aq,
SB-C3 and SB-Phenyl)
InfinityLab Poroshell HPH-C18 and
HPH-C8
pH 3.0 to 11.0 60 °C
ZORBAX Extend C18 pH 2.0 to 11.5 60 °C (40 °C at high pH)
Note: All silica-based packings have some solubility in pH >6 aqueous mobile
phases. When using silica-based columns at pH >6, best column lifetime is
obtained at lower temperatures (40 °C max) using low buffer concentrations in the
range of 0.01 to 0.02 M. Operating at extreme ends of pH and temperature ranges
will have a significant impact on column lifetime.
ENGLISH
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