Recommended Starting Gradients
Stationary phase Usage notes
Most reversed-phase
columns
5% methanol or acetonitrile initially and 100%
methanol or acetonitrile as the final solvent.
ZORBAX Eclipse PAH* 30 or 40% acetonitrile initially, to 100%
acetonitrile as final solvent. It may be necessary
to cool column to 15 to 20 °C for improved
resolution.
ZORBAX and InfinityLab
Poroshell Bonus-RP
Lower concentration of organic mobile phase
modifier is required for compound elution,
compared to traditional long-chain alkyl
stationary phases.
*Best column lifetime is achieved at < 40 °C.
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