Storage Recommendations
Long-term storage of silica-based, bonded phase columns should be in
a pure organic solvent. If the column has previously been used with a
buffered mobile phase, the buffer should first be removed by purging the
column with 20 to 30 column volumes of a 50:50 mixture of methanol or
acetonitrile and water, followed by 20 to 30 column volumes of the pure
solvent. Before storing, end-fittings should be tightly capped with end-
plugs to prevent packing from drying out. Columns may be safely stored for
short periods in most mobile phases. To protect equipment, is it desirable
to remove salts from the instrument and column by purging the column
with the same mobile phase without the buffer (e.g. using 60:40 ACN/
H
2
0 to remove a 60:40 ACN/0.02 M phosphate buffered mobile phase).
Re-equilibration is rapid with the original mobile phase when using this
approach and any danger of corrosion from the salts is eliminated.
ENGLISH
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