Perfusion Pressure Kit
(Sold separately)
Hoses should continue from syringes to valves.
Syringes may also be placed in a water
bath for temperature control.
Brass input connector hose barb size 1/4”
5 micron bowl lter.
Press bottom button to empty.
Be very careful with these
plastic hose connections.
Press regulator knob down to lock.
Pull up to unlock.
Individual air lines can be
adjusted or turned-o.
2-way stopcocks and luer-lock to
1
/
16
” hose ttings are
included for each channel to connect syringes to hose.
Max. input
pressure 100 psi!!
Perfusion Pencil Multi-barrel Manifold
(Sold separately)
Tighten removable tip for
minimum dead volume.
One of the eight lines may be connected to
vacuum through a valve programmed to suck
the dead-volume clear between solutions.
Chemical Information
The tubing inside the manifold body is polyimide (nylon). The removable tips
include a polycarbonate luer lock tting with a fused silica (quartz) needle coated
with polyimide. These materials are resistant to most acids, bases and organic
solvents. To avoid dust contamination, we recommend pre-rinsing the Perfusion
Pencil and tip with distilled water. Also, it is good practice to discard the rst few
microliters of solution before using the device. The Perfusion Pencil and removable
tips are shipped non-sterile. They can be chemically sterilized or autoclaved.
However, repeated autoclaving may weaken the adhesive bond between the luer
lock tting and the needle.
Maintenance
You can expect several years of useful lifetime for your tip if you wash it daily.
Use a syringe or vacuum to pull water, then alcohol three times each through the
tip. If the Perfusion Pencil is lled with pure salt solution, leaving it in the syringe
overnight will not usually cause the tip to block. However, if it is used for high
viscosity uids, ushing after each use is recommended.
Cutting
The removable tips are shipped with 0.5" (1 cm) length polyimide needles.
They can be cut shorter by rolling a razor blade on them against a hard surface.
However, this may leave a small crack or barb on the tip. To get a at cut, score the
coating of the needle with a ceramic cleaving stone or a diamond cutter and pull
directly apart, making sure not to pull at an angle. You may notice a larger outer
tube enclosing the lower part of our smaller, 100µm needles. This is simply for
added rigidity, and can be cut if needed.
Replacement Tips:
#04-xxx Specify i.d. size:
100, 250, or 360µm
All inow tubes must be lled with solution
before use even if some lines are not needed, or
liquids will backow into empty lines!
Part No.
#04-04-xxx 4-into-1 Pencil with tip
#04-08-xxx 8-into-1 Pencil with tip
#04-16-xxx 16-into-1 Pencil with tip
(xxx = 100, 250, or 360µm)
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