BD FACSAria Startup, Qc & Shutdown Procedure

1 Aria-2/4: Turn on Aerosol Management System
Aria-7: Turn on Class 1 BSC by turning Fans on
and set AMS fan to 20%
2 The AMS should be operating at 20% at all times
under normal conditions. If clog occurs  See Cell
Sorter Clog SOP for further instructions.
1 Open Air valve for Aria-2/4. Turn yellow lever on wall until it’s parallel with the pipe.
This is not required for Aria-7.
1
Stream/Sort
QC
Accudrop
FACSAria™
Startup, QC & Shutdown Procedure
Ver.
2019-12-22
Workflow Overview
Daily Startup Procedure for FACSAria™ Sorters
Turn ON
Hardware
Software
Fluidics
&
Stream
Startup
Cleaning
Sort Chamber
Optics
QC
1. Turning On Hardware/Software
2
Post Sort
Cleanup/
Shutdown
AMS On/Off
switch
100% evacuation
button
1
2
1
2

BD FACSAria Specifications

General

Sorting Speed	Up to 70, 000 events per second
Laser Options	488 nm, 633 nm, 405 nm
Fluorescence Detectors	PMT detectors
Sample Collection	Tubes or plates
Nozzle Sizes	70 μm, 85 μm, 100 μm, 130 μm
Cell Size Range	1-50 μm
Sorting Modes	Single cell, bulk sort, index sorting
Data Acquisition	BD FACSDiva software