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BD FACSAria II User Manual

BD FACSAria II
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BD FACSAria II Quick Reference Guide
Use this quick reference guide once you are familiar with the procedures in
the BD FACSAria II User’s Guide.
Starting Up the Cytometer
Follow these steps to start up your BD FACSAria™ II cytometer. For more
details, refer to Chapter 4 in the user’s guide.
1 Start up the computer.
2 Turn on the cytometer main power and the lasers. Wait 30 minutes for
the lasers to warm up.
;Tip If you are using temperature
control, start heating or cooling the
sample injection chamber or the
recirculating water bath while the
lasers are warming up.
3 Start BD FACSDiva™ software and log in with your user
name and password.
4 Verify fluidics levels in the Cytometer window. Replenish
fluids or empty the waste, if needed.
5 Select Cytometer > Fluidics Startup.
6 Follow the instructions in the Fluidics Startup wizard to complete the
process.
Verify that the air and fluid lines are connected to the sheath tank, then
click Done.
main power
sheath waste bleachDI ethanol

Table of Contents

Questions and Answers:

BD FACSAria II Specifications

General IconGeneral
BrandBD
ModelFACSAria II
CategoryLaboratory Equipment
LanguageEnglish

Summary

Starting Up the Cytometer

Start up the computer

Initiate the computer system before powering on the cytometer.

Turn on main power and lasers

Power on the cytometer and its lasers, allowing 30 minutes for warm-up.

Verify and replenish fluidics

Check fluid levels in the Cytometer window and address any needs.

Initiate Fluidics Startup

Navigate to Cytometer > Fluidics Startup to begin the process.

Complete Fluidics Startup wizard

Follow the on-screen wizard instructions for fluidics setup.

Start the stream

Activate the stream by clicking the Stream button in the Breakoff window.

Check stream position and image

Inspect the stream in the waste aspirator and Breakoff window for normalcy.

Adjust Amplitude for drop merging

Fine-tune the Amplitude setting for optimal satellite drop merging.

Sorting Setup

Check laser delay and scaling factors

Calculate drop delay using Accudrop

Sorting

Verify tube pointer and start sort

Confirm aspirator/deflection plates

Shutting Down the Cytometer

Turn off the stream

Remove nozzle and install closed-loop nozzle

Clean the flow cell

Select cleaning mode and use the specified cleaning solution.

Perform daily shutdown steps

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