EasyManua.ls Logo

BD FACSCanto User Manual

BD FACSCanto
184 pages
To Next Page IconTo Next Page
To Next Page IconTo Next Page
Page #1 background imageLoading...
Page #1 background image
bdbiosciences.com
Part No. 642224 Rev. A
April 2007
BD Biosciences
2350 Qume Drive
San Jose, CA 95131-1807
USA
Tel (877) 232-8995
Fax (408) 954-2347
Brazil
Tel (55) 11-5185-9995
Fax (55) 11-5185-9895
Europe
Tel (32) 2 400 98 95
Fax (32) 2 401 70 94
Mexico
Toll Free 01-800-236-2543
Tel (52) 55 5999 8296
Japan
Nippon Becton Dickinson Company, Ltd.
Toll Free 0120-8555-90
Asia Pacific
Fax (65) 6-860-1590
Canada
Toll Free (888) 259-0187
Fax (888) 229-9918
Tel (65) 6-861-0633
Tel (905) 542-8028
canada@bd.com
help.biosciences@europe.bd.com Fax (52) 55 5999 8288Tel 81-24-593-5405
Fax 81-24-593-5761
bdbiosciences.com
Part No. 642224 Rev. A
June 2007
BD Biosciences
2350 Qume Drive
San Jose, CA 95131-1807
USA
Tel (877) 232-8995
Fax (800) 325-9637
Brazil
Tel (55) 11-5185-9995
Fax (55) 11-5185-9895
Europe
Tel (32) 2 400 98 95
Fax (32) 2 401 70 94
Mexico
Toll Free 01-800-236-2543
Tel (52) 55 5999 8296
Japan
Nippon Becton Dickinson Company, Ltd.
Toll Free 0120-8555-90
Asia Pacific
Fax (65) 6-860-1590
Canada
Toll Free (888) 259-0187
Fax (888) 229-9918
Tel (65) 6-861-0633
Tel (905) 542-8028
canada@bd.com
facservice@bd.com
help.biosciences@europe.bd.com Tel 81-24-593-5405
Fax 81-24-593-5761
Fax (52) 55 5999 8288
BD High Throughput
Sampler User’s Guide
for the
BD LSR II
BD FACSCanto
BD FACSCanto II

Table of Contents

Other manuals for BD FACSCanto

Question and Answer IconNeed help?

Do you have a question about the BD FACSCanto and is the answer not in the manual?

BD FACSCanto Specifications

General IconGeneral
Detection ChannelsUp to 8 fluorescence channels
Data ManagementBD FACSDiva software
Forward Scatter DetectorPhotodiode
Laser OptionsBlue (488 nm), Red (633 nm), Violet (405 nm)
Sample Input12 x 75 mm tubes
ApplicationsImmunophenotyping, Apoptosis
Side Scatter DetectorPhotomultiplier tube
Sample TypesWhole blood
Power Requirements100-240 VAC, 50/60 Hz

Summary

About This Guide

Chapter 1: Introduction

HTS Hardware and Components Overview

Covers the HTS hardware functionality, components, and throughput modes.

Cytometer Connections and Setup

Explains HTS unit connection to the cytometer and initial setup steps.

Sample Processing and Modes

Compares sample acquisition sequences in standard and high-throughput modes.

Chapter 2: BD FACSDiva Software Overview

Workspace and Plate Window Navigation

Describes the BD FACSDiva workspace, components, and Plate window views.

Plate Setup and Configuration

Details Plate window components, toolbar, layout, loader settings, and functions.

Acquisition Modes and Procedures

Explains automatic and manual acquisition modes and their procedures.

Analysis View and Data Analysis

Covers Analysis view components, functions, layout, and keyword display.

Performing Batch Analysis

Explains how to use the batch analysis feature for automated data analysis.

Chapter 3: Running Samples

System Startup and Experiment Creation

Guides on starting the system, creating folders, and new experiments.

Plate and Worksheet Setup

Details setting up plates, worksheets, and exporting templates for experiments.

Acquisition Preparation and Execution

Covers preparing for acquisition, compensation, acquiring data, pausing, and stopping.

Experiment Export and Data Analysis

Guides on exporting experiments as templates and analyzing collected data.

Data Maintenance and Mode Switching

Covers data maintenance, and switching between plate and tube acquisition modes.

System Shutdown and Software Quitting

Outlines procedures for shutting down the system and quitting the software.

Chapter 4: Maintenance

Daily Maintenance Procedures

Covers daily cleaning, inspection, and servicing of the HTS unit.

Monthly Maintenance Procedures

Details monthly cleaning, inspection, and servicing for HTS unit upkeep.

Periodic Component Replacement

Lists components for periodic replacement and their procedures.

Unscheduled Maintenance and Troubleshooting Procedures

Covers troubleshooting steps like homing, priming, motion tests, and fittings inspection.

Chapter 5: Troubleshooting

HTS Troubleshooting

Table of observations, causes, and solutions for HTS-specific issues.

Acquisition Troubleshooting

Table of observations, causes, and solutions for acquisition-related problems.

BD FACSDiva Troubleshooting

Table of observations, causes, and solutions for software-related issues.

Appendix A: Consumables and Replacement Parts

Cytometer Supplies and Maintenance Log

Lists HTS unit supplies, replacement parts, and a maintenance log template.

Appendix B: Depot Repair Procedures

Depot Repair Overview and Unit Removal

Outlines the depot repair process and procedures for removing the HTS unit.

HTS Unit Cleaning and Decontamination

Procedures for cleaning and decontaminating the HTS unit before repair or shipping.

Unit Packing, Unpacking, and Installation

Guides on packing, unpacking, and installing a replacement HTS unit.

Related product manuals