138 BD High Throughput Sampler User’s Guide
Priming the HTS
Prime the HTS unit when you set it up for the first time (eg, after a depot repair
procedure), when you notice bubbles in the pump syringe barrel, or to clear a
clog in the HTS fluidics. The Prime command automatically primes both pumps.
1 [BD LSR II] Ensure that the sample coupler is installed, and place the
cytometer in Run mode.
To prevent pressure from building in the flow cell, the cytometer must be in
Run mode before priming begins. If you try to run the Prime command
when the cytometer is not in Run mode, a software message will remind
you that the fluidic system is not ready.
[BD FACSCanto and BD FACSCanto II] Ensure the sample coupler is
installed and the HTS is turned on.
2 Choose HTS > Prime.
3 Verify that the pumps are primed and the HTS tubing fills with sheath fluid.
Once priming is complete, the following message appears.
4 Click OK to dismiss the message.
Performing a Motion Test
You might need to perform a motion test when you set up the HTS unit for the
first time (ie, after a depot repair procedure), when axis movement is suspect, or
when you are directed to do so by a BD Biosciences service representative during
cytometer troubleshooting.
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