Chapter 1: Introduction 19
Cytometer Connections
Cytometer Interface Panel
Sheath and waste travel between the cytometer and the HTS unit via connectors
in the cytometer interface panel (see Figure 1-5, Figure 1-6, and Figure 1-7).
The BD LSR II interface panel also includes an acquisition mode switch, which
controls pressure at the cytometer sample injection tube (SIT).
• In Tube mode ( ), the droplet containment module (DCM) vacuum
functions normally—the vacuum is on when the arm is positioned to the
side and off when the arm is centered. Backdripping from the sample
injection tube is contained when the DCM sleeve is installed.
• In Plate mode ( ), the DCM vacuum on the cytometer does not function.
Drip containment is provided by the absorbent pad in back of the HTS unit
(Figure 1-3 on page 17).
[BD LSR II] To keep your cytometer free of drips from potentially
biohazardous samples, always switch the cytometer to Tube mode and
install the DCM sleeve when you are not acquiring samples using the HTS
option. Note that if backdripping does occur, drips are contained by the
absorbent pad in back of the HTS unit.
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