Chapter 5: Troubleshooting 149
BD FACSDiva Troubleshooting
Observation Possible Causes Recommended Solutions
System error Cover error Replace cover and resume run.
Back pressure error • Ensure waste line to cytometer
is properly installed and not
kinked.
• Ensure filter on waste tank is
not clogged.
Motion error Select HTS > Home and resume
run.
Pump error Select HTS > Home and resume
run.
Plots not refreshing Incorrect worksheet displayed Ensure global worksheet is
displayed during a sequenced,
plate-based acquisition.
Run buttons not enabled No global worksheet selected Ensure a global worksheet is
selected in the global worksheet
folder.
Well(s) not selected Select a well(s).
[BD FACSCanto and
BD FACSCanto II only]
HTS not included in
cleaning modes
Sample coupler not installed Ensure sample coupler is installed
before cleaning modes are
selected.
No power Ensure HTS is powered on.
Cover open Close cover.
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