104 BD High Throughput Sampler User’s Guide
Daily Maintenance
When running the HTS daily, perform the following at the end of every day (or
shift):
• Daily Cleaning—see the following section
• Cytometer Inspection and Servicing—see page 109
NOTICE [BD LSR II] When running samples containing acridine orange or
propidium iodide, run Daily Clean twice. First, run 70% isopropyl alcohol in
wells A1
–A4 and BD™ FACSClean or a 10% bleach solution in wells B1–B4.
Second, run BD™ FACSRinse solution in wells A1–A4, followed by DI water in wells
B1–B4.
Daily Cleaning
During the daily clean procedure, the cytometer samples cleaning solution and
then DI water from predefined wells and performs a sequence of mixing,
aspiration, and rinsing. Software prompts guide you through the cleaning
sequence. Perform the cleaning procedure at the end of every day or shift while
the cytometer is in plate-based mode. Allow 15 minutes to complete this
procedure.
Materials Needed
• BD FACSClean or a fresh 10% bleach solution (1 part bleach in 9 parts DI
water)
• BD FACSRinse solution
• DI water
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