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BD Accuri C6 User Manual

BD Accuri C6
31 pages
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BD Accuri C6 Specifications

General IconGeneral
Sample Acquisition RateUp to 10, 000 events/second
Sample Flow Rate35 μL/min
Operating Temperature15°C to 30°C
Power Requirements100–240 VAC; 50/60 Hz
Fluorescence Channels4 (FL1, FL2, FL3, FL4)

Summary

Introduction

About This Manual

Provides information on cytometer requirements, setup, maintenance, and troubleshooting.

Delivery and Inspection

Instructions for inspecting the cytometer upon receipt for signs of damage or mishandling.

BD Accuri C6 Flow Cytometer Specifications

Details technical specifications of the BD Accuri C6 Flow Cytometer, including lasers, optics, and performance.

Safety Precautions

Safety and Limitations Manual

Refers to a separate manual for comprehensive safety information and instrument limitations.

Operational Considerations

Discusses environmental and usage conditions for optimal cytometer performance.

Ambient Temperature and Humidity

Specifies the acceptable operating temperature range (15-30°C) and humidity (<80%).

Area of Use

Describes recommended placement and ventilation requirements for the cytometer on a lab bench.

Approved Reagents

Lists the recommended fluids and solutions for operation and cleaning of the instrument.

Material Safety Data Sheets (MSDS)

Provides contact information and website to obtain Material Safety Data Sheets (MSDS).

Setting Up the BD Accuri C6 Flow Cytometer

BD Accuri C6 Flow Cytometer Layout

Identifies and illustrates the major components of the fluidics and optics subsystems of the cytometer.

Configuring the Computer Settings

Guides on initial setup of the computer, monitor, keyboard, and mouse for the cytometer.

Setting up the Computer

Basic steps for setting up the computer, monitor, keyboard, and mouse according to manufacturer instructions.

Changing the Workstation Computer Settings for Windows® 7

Optimizes Windows 7 power and display settings for the BD Accuri C6 workstation.

Viewing .c6 and .c6t File Extensions in Windows 7

Instructions to show hidden file extensions in Windows 7 for BD Accuri C6 software files.

Setting Up the BD Accuri C6 Flow Cytometer

Procedures for physically setting up the BD Accuri C6 Flow Cytometer, including cover and filter checks.

Setting Up the Reagent Bottles and Fluidics

Details on filling reagent bottles and connecting fluidics harness to the cytometer and bottles.

Connecting the Cables

Instructions for connecting the power supply and USB cables to the cytometer and computer.

Installing Software

Information on installing the BD Accuri C6 Software and the cytometer driver, if not pre-installed.

Installing BD Accuri C6 Software

Step-by-step guide for installing the BD Accuri C6 Software using the provided CD.

Starting Up the BD Accuri C6 Flow Cytometer

Procedure for powering on the cytometer, initial fluidics flush, and validation.

Turning Off the BD Accuri C6 Flow Cytometer

Detailed steps for properly shutting down the BD Accuri C6 Flow Cytometer via software and power button.

Maintenance

Automated Preventive Maintenance Notification

Describes the system's automated maintenance reminders triggered by usage or time.

Cleaning the SIP

Procedure for cleaning the Sample Introduction Port (SIP) to remove clogs using a backflush cycle.

Cleaning the Flow Cell

Instructions for cleaning the flow cell as part of regular maintenance or to correct performance issues.

Running the Unclog Cycle

Steps to purge the flow cell of debris using the unclog cycle function.

Running an Extended Clean of the Flow Cell

Procedure for a thorough flow cell cleaning cycle, including a soak period.

Decontamination Fluid Cycle

Details the process and timing for the cytometer's decontamination fluid cycle.

Cleaning Fluid Cycle

Procedure for running the cleaning fluid cycle to flush the system with cleaner fluid.

Purging Air from the BD Accuri C6 Flow Cytometer

Steps to remove air from the fluidic system, relevant for first-time setup or after extended non-use.

Emptying the Waste Container

Instructions for safely emptying the waste bottle daily or when prompted by the software.

Filling the Fluid Bottles

Guidance on checking and refilling sheath, cleaning, and decontamination fluid bottles.

Replacing the Fluidic Bottle Filters

Steps for replacing the disk filters in the sheath, cleaner, and decontamination fluid bottles.

Inspecting the Fluidics Lines

Procedure for checking fluidics lines for leaks, crimping, or dried residue.

Replacing the In-line Sheath Filter

Instructions for replacing the in-line sheath filter, recommended every two months.

Replacing the Peristaltic Pump Tubing

Steps for replacing the peristaltic pump tubing for both sheath and waste pumps.

Troubleshooting and Technical Support

Troubleshooting

General guidelines for resolving common instrument issues, with contact info for persistent problems.

Cleaning and Decontamination—Standard Procedure

Procedure for cleaning and decontaminating the system, recommended for storage or service.

Limited Warranty

Information on the product's limited warranty period, coverage for software/firmware, and exclusions.

Extended Warranties

Information on the availability and details of purchasing extended warranty coverage for the product.

Technical Support Contact Information

Contact details and locations for obtaining technical support in USA, Europe, Middle East, Africa, and Canada.

Ordering Information

Information on how to order replacement parts and reagents, including regional office contacts.

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