BD Accuri C6 - BD Accuri C6 Flow Cytometer Specifications

BD Accuri C6 Flow Cytometer Instrument Manual
2 7820018-01 Rev-2
Table 1-3. BD Accuri C6 Flow Cytometer Specifications
Laser Excitation
488 nm (rated at 20,000 hr life)
640 nm (rated at 20,000 hr life)
Laser Profile
10 x 75 µm
Laser Power
14.7 mW 640 nm Diode Red Laser
20 mW 488 nm Solid State Blue Laser
Scatter Detection
Forward (0 degrees, +/-13)
Side (90 degrees, +/-13)
Emission Detection
4 colors, standard optical filters
 FL1 533/30 nm (e.g. FITC/GFP)
 FL2 585/40 nm (e.g. PE/PI)
 FL3 > 670 nm (e.g. PerCP, PerCP-Cy™5.5, PE-Cy7)
 FL4 675/25 nm (e.g. APC)
Optical Alignment
Fixed alignment
Flow Cell
200 µm ID quartz capillary
Minimum Detectable Particle
Size
0.5 µm
Minimum Sample Volume
300 µL (in 12 x 75 mm tube)
Pre-Set Flow Rates and Core
Sizes
Slow: 14 µL/min, 10 µm core
Medium: 35 µL/min, 16 µm core
Fast: 66 µL/min, 22 µm core
Custom Sample Flow Rates
10 – 100 µL/min
Custom Core Diameter
5 – 40 µm
Recommended Sheath Fluid
0.22 µm filtered, deionized (DI) water
Maximum Events Per Sample
1 million events per well
Florescence Sensitivity,
MESF*
FITC < 150; PE < 100
Scatter Resolution
Resolves human peripheral blood lymphocytes,
monocytes, and granulocytes
Fluorescence Linearity
2 ± 0.05% for chick erythrocyte nuclei (CEN)
Fluorescence Precision
≤ 3% CV for CEN
Data Acquisition Rate
10,000 events/second, maximum
Power Requirements
100 - 240 VAC, 50/60 Hz
Typical Power Consumption
154 VA
Heat Output
240 Btu/hour maximum output
Operating Ranges
15 - 30° C; < 80% relative humidity
Instrument Size
11”H x 14.75”W x 16.5”D (27.9 x 37.5 x 41.9 cm)
Foot Print with Fluid Bottles
11”H x 21.5”W x 16.5”D (27.9 x 54.6 x 41.9 cm)
Weight
30 lbs (13.6 kg)
Fluid bottle capacity
2 L sheath fluid, 2 L waste,
250 mL cleaner, 250 mL decontamination
Signal Processing
24-bit datapath
Computer Interface
USB 2.0
Warranty
1 year
*MESF values determined using Thermo Scientific Cyto-Cal™ Multifluor Plus Violet Beads.