BD Accuri C6 - Table of Contents

BD Accuri C6 Flow Cytometer Instrument Manual
7820018 Rev-2 i
TABLE OF CONTENTS
1 INTRODUCTION ................................................................................................................. 1
1.1 About This Manual ..................................................................................................... 1
1.2 Delivery and Inspection ............................................................................................. 1
1.3 BD Accuri C6 Flow Cytometer Specifications ............................................................. 2
2 SAFETY PRECAUTIONS .................................................................................................... 3
2.1 Safety and Limitations Manual ................................................................................... 3
2.2 Operational Considerations ....................................................................................... 3
2.2.1 Ambient Temperature and Humidity ............................................................... 3
2.2.2 Area of Use .................................................................................................... 3
2.2.3 Approved Reagents ....................................................................................... 3
2.2.4 Material Safety Data Sheets (MSDS) ............................................................. 4
3 SETTING UP THE BD ACCURI C6 FLOW CYTOMETER ................................................... 5
3.1 BD Accuri C6 Flow Cytometer Layout ........................................................................ 5
3.2 Configuring the Computer Settings ............................................................................ 5
3.2.1 Setting up the Computer ................................................................................ 5
3.2.2 Changing the Workstation Computer Settings for Windows
®
7 ....................... 6
3.2.3 Viewing .c6 and .c6t File Extensions in Windows 7 ........................................ 6
3.3 Setting Up the BD Accuri C6 Flow Cytometer ............................................................ 7
3.3.1 Setting Up the Reagent Bottles and Fluidics .................................................. 8
3.3.2 Connecting the Cables ................................................................................... 9
3.4 Installing Software ................................................................................................... 10
3.4.1 Installing BD Accuri C6 Software .................................................................. 10
3.5 Starting Up the BD Accuri C6 Flow Cytometer ......................................................... 10
3.6 Turning Off the BD Accuri C6 Flow Cytometer ......................................................... 11
4 MAINTENANCE ................................................................................................................. 13
4.1 Automated Preventive Maintenance Notification ...................................................... 13
4.2 Cleaning the SIP ...................................................................................................... 14
4.3 Cleaning the Flow Cell ............................................................................................. 14
4.3.1 Running the Unclog Cycle ............................................................................ 14
4.3.2 Running an Extended Clean of the Flow Cell ............................................... 15
4.4 Decontamination Fluid Cycle ................................................................................... 15
4.5 Cleaning Fluid Cycle ................................................................................................ 15
4.6 Purging Air from the BD Accuri C6 Flow Cytometer ................................................. 16
4.7 Emptying the Waste Container ................................................................................ 16
4.8 Filling the Fluid Bottles ............................................................................................. 17
4.9 Replacing the Fluidic Bottle Filters ........................................................................... 17
4.10 Inspecting the Fluidics Lines .................................................................................... 18
4.11 Replacing the In-line Sheath Filter ........................................................................... 18
4.12 Replacing the Peristaltic Pump Tubing .................................................................... 19
5 TROUBLESHOOTING AND TECHNICAL SUPPORT ........................................................ 21
5.1 Troubleshooting ....................................................................................................... 21
5.2 Cleaning and Decontamination—Standard Procedure ............................................. 24
5.3 Limited Warranty ..................................................................................................... 25
5.4 Extended Warranties ............................................................................................... 25
5.5 Technical Support .................................................................................................... 25
5.6 Ordering Information ................................................................................................ 27