BD Accuri C6 Flow Cytometer Instrument Manual
7820018 Rev-2 11
4. Firmly press the power button on the front of the unit to turn on the cytometer.
During start up, the BD Accuri C6 Software Traffic Light turns yellow and the
cytometer pumps start to run. When powered on, the cytometer automatically
flushes the fluidics lines with sheath. This process takes approximately five
minutes.
Do not open the lid of the cytometer during the startup process. Opening
the lid interrupts the laser warm-up and extends the time before data can be
acquired.
5. When the BD Accuri C6 Software Traffic Light turns green and BD Accuri C6
Software displays the message C6 is connected and ready, run 0.22 µm filtered,
DI water for at least 15 minutes before processing samples.
6. Validate cytometer performance. See the BD Accuri C6 Software User’s Guide
for details on instrument validation using the validation beads provided with the
cytometer.
1. Place a tube with 2 mL of decontamination solution on the SIP.
2. Select an empty data well in the Collect Tab of BD Accuri C6 Software.
3. Set the time limit for two minutes and the fluidics speed to fast.
4. Click on the RUN button.
5. Once the run is finished, remove the tube of decontamination solution from the
SIP.
6. Place a tube with 2 mL of 0.22 µm filtered, DI water on the SIP and advance to
any empty data well.
7. Set the time limit for two minutes and fluidics to Fast.
8. Click on the RUN button.
9. When the run is finished, leave the tube on the SIP.
10. Press the power button. The shutdown cycle runs for approximately 15 minutes,
then the cytometer automatically powers off.
Depressing the power button for five seconds or longer bypasses the auto-
fluidic shutdown cycle. If the cytometer is shut down in this manner, BD Accuri
C6 Software displays the following message during the next start up:
Figure 3-8. Improper Shut down Message
When this occurs, the cytometer takes additional time to recover and return to
the green light ready state.
To Next Page
Loading...
