BD Accuri C6 Flow Cytometer Instrument Manual
7820018 Rev-2 15
Click on the Unclog button in the Collect tab.
Select Instrument > Run Unclog Cycle.
For a thorough cleaning of the flow cell, perform an extended flow cell clean. During
extended flow cell cleaning, the flow cell fills completely with cleaning solution from
the sample tube on the SIP. This cycle automatically shuts down the cytometer with
cleaning reagent in the flow cell, allowing the flow cell to soak.
1. Place a tube with at least 500 µL of Extended Flow Cell Clean Solution (PN
653159) on the SIP.
Never run the Extended Clean of Flow Cell cycle without a tube containing
at least 500 µL of fluid.
2. Select Instrument > Extended clean of flow cell.
3. After the cytometer is automatically shut down, allow the cytometer to rest for at
least 30 minutes (up to overnight for a more thorough cleaning).
4. Replace the tube of Extended Flow Cell Clean Solution with a tube of 2 mL 0.22
µm filtered, DI water.
5. Restart the cytometer. The cytometer performs a longer fluidics startup cycle and
the Software displays the message Extra startup time needed due to cleaning or
improper shutdown. This longer cycle purges cleaning reagent from the flow cell.
6. Run 0.22 µm filtered, DI water for 10 minutes on fast speed.
7. Operate the cytometer as usual.
The BD Accuri C6 automatically runs the decontamination fluid cycle when the
system is shut down normally. The decontamination fluid cycle lasts about 15
minutes.
To manually run the decontamination fluid cycle:
1. Place a tube with 2mL of 0.22 µm filtered, DI water on the SIP.
2. Select Instrument > Run decontamination fluid cycle.
The cleaning fluid cycle pulls cleaner fluid from the cleaner tank and runs it through
the fluidic lines of the fluidics system. After filling the system with cleaner fluid, the
cleaning fluid cycle purges the cytometer with fresh sheath fluid and performs a
backflush. This cycle takes about five minutes.
1. Place a tube of cleaning solution (PN 653157) on the SIP.
2. Select Instrument > Run cleaning fluid cycle.
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