BD Accuri C6 Flow Cytometer Instrument Manual
16 7820018-01 Rev-2
Purge air from the cytometer if any of the following is true:
The cytometer is being set up for the first time.
The cytometer has not been used for more than two days.
A sample has been run dry or the cytometer has been running without sheath in
the sheath bottle.
To purge air from the cytometer:
1. Place a tube containing sheath on the SIP.
NOTE: If performing an air purge at any time other than initial setup, skip steps
2-3.
2. Select Instrument > Run decontamination fluid cycle.
3. Wait approximately 15 minutes for the decontamination fluid cycle to complete.
4. Set the run time in the software control panel to five minutes and click on the Run
button. Allow the cytometer to run until it stops automatically.
NOTE: The peristaltic pumps will make a rhythmic sound while running. This is
normal.
5. Click on the Run button again, wait for 30 seconds, and click on the Pause button
to stop the pumps.
6. Repeat step 5 two more times to purge bubbles from the system.
Empty the waste bottle daily or when prompted by BD Accuri C6 Software to prevent
spillover.
Biological samples are potentially dangerous and/or life threatening. Adhere to
proper handling procedures for samples and reagents at all times. Wear appropriate
laboratory attire such as protective gloves and eyewear during this procedure.
1. Disconnect the quick connect waste line from the top of the waste bottle.
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