BD Accuri C6 Flow Cytometer Instrument Manual
7820018 Rev-2 19
8. Gently close the cytometer cover.
9. Place a sample tube with 2mL of 0.22 µm filtered, DI water on the SIP.
10. Plug in and turn on the cytometer.
11. Purge any air from the instrument (see section 4.6).
Replace the pump tubing (PN 653146) every two months. Replace both pieces of
tubing at the same time. Tubing comes in contact with biological samples and
therefore should be considered hazardous. Wear gloves and appropriate safety gear
during this procedure.
There are two peristaltic pumps: a sheath pump and a waste pump.
1. Turn off and unplug the cytometer.
2. Lift the cytometer lid.
3. Disconnect the luer locks from the peristaltic pumps by unscrewing them. Blue
tubing is connected to the sheath pump and red tubing to the waste pump.
Figure 4-4. Peristaltic Pumps and Luer Locks
4. Squeeze the grip marks on the pump element retainer clip to remove the clip.
Figure 4-5. Removing Peristaltic Pump Retainer Clip
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