BD Accuri C6 Flow Cytometer Instrument Manual
8 7820018-01 Rev-2
6. Gently close the cytometer cover. Make sure the cover is securely positioned. If
the cover is not closed securely, the cytometer will not function.
7. Place the fluidics bottle tray in a convenient location near the perimeter of the
cytometer.
Do not place the fluidics bottle tray on top of the cytometer.
1. Fill the sheath bottle with sheath fluid.
2. Dilute the decontamination concentrate (PN 653154) and cleaning concentrate
(PN 653157) solutions as instructed and fill decontamination and cleaner bottles,
respectively.
Close the bottles securely. Do not over tighten.
3. Add approximately 100 mL of 0.5% NaOCl to the Waste bottle.
4. Place the bottles in the fluidics bottle tray.
Figure 3-4. Fluidics harness
5. Slide the red tab on the black end of the fluidics harness to the unlock position
and attach the black end of the fluidics harness to the back of the cytometer.
6. Slide the red tab to the lock position.
7. Attach the other end of the fluidics harness to the appropriate bottles:
Red line—waste bottle
Blue line—sheath bottle
Yellow line—decontamination bottle
Green line—cleaner bottle
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