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BD LSRFortessa User Manual

BD LSRFortessa
20 pages
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bdbiosciences.com
23-11093-00 Rev. A
3/2010
For Research Use Only
Becton, Dickinson and Company
BD Biosciences
San Jose, CA 95131
Tel 877.232.8995
Fax 800.325.9637
ResearchApplications@bd.com
BD Biosciences
European Customer Support
Tel 32.2.400.98.95
Fax 32.2.401.70.94
help.biosciences@europe.bd.com
BD LSRFortessa
Cell Analyzer
User’s Guide

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BD LSRFortessa Specifications

General IconGeneral
Side Scatter (SSC)PMT
Forward Scatter DetectorPhotodiode
LasersUp to 5 lasers
SoftwareBD FACSDiva software
Laser OptionsBlue (488 nm), Red (633 nm), Violet (405 nm), Yellow-Green (561 nm), UV (355 nm)
DetectorsPMTs (Photomultiplier Tubes)
Forward Scatter (FSC)Photodiode
Sample InputTube
Laser ConfigurationUser-configurable
Fluorescence DetectorsPMTs
Nozzle Size70, 85, 100 µm
Power Requirements100-240 VAC, 50/60 Hz

Summary

About this guide

What this guide covers

Describes the procedures to operate and maintain the BD LSRFortessa™ cell analyzer and its software.

Conventions used in this guide

Explains the conventions used throughout the guide, including safety symbols and text formatting.

BD LSRFortessa Documentation

Details the available documentation, including publication formats and online help resources.

Technical Assistance

Provides guidance on obtaining technical support and troubleshooting assistance for the instrument.

Introduction

Instrument Overview

Provides a general description of the BD LSRFortessa™ flow cytometer and its core technology.

Instrument Components

Details the various physical components of the BD LSRFortessa™ instrument, including ports and panels.

Control Panel Operations

Describes the controls located on the instrument's front panel, including fluidics controls.

Cytometer Setup

Starting the Cytometer and Computer

Instructions for initiating the cytometer and associated computer systems for operation.

Preparing the Sheath Container

Guidance on filling and preparing the sheath fluid container for the cytometer.

Removing Air Bubbles

Steps for effectively removing air bubbles from the fluidic system.

Preparing the Waste Container

Instructions for preparing the waste fluid container.

Priming the Fluidics

Procedure for priming the fluidic system to ensure proper flow.

Optical Filters and Mirrors

Information regarding the optical filters and mirrors within the cytometer.

Changing Optical Filters and Mirrors

Steps for the replacement of optical filters and mirrors.

Custom Configurations and Baselines

Guidance on creating custom instrument configurations and baselines.

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