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FACSCanto II
BD FACSCanto II User Manual
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bdbiosciences.com
Part No. 642239
Rev
.
A
June 2007
BD Bioscienc
es
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F
or In Vitro Diagnostic Use
BD
F
ACSCanto
II
Instructions For Use
2
Table of Contents
Default Chapter
5
Table of Contents
5
About These Instructions
11
Conventions
12
Chapter 1: Introduction
13
System Components and Theory of Operation
14
Flow Cytometer Components
15
Fluidics Cart
22
BD FACS Loader (Optional)
25
Barcode Reader (Optional)
27
System Requirements
28
Chapter 2: Software Windows and Toolbars
31
BD Facscanto Clinical Software Workspace
32
BD Facscanto Toolbars
33
BD Facsdiva Software Workspace
34
Chapter 3: Barcode Reader Option
39
Installing and Using the Barcode Reader
40
Cleaning the Barcode Reader
42
1D Barcode Symbologies
43
2D Barcode Symbologies
44
Chapter 4: Starting up
45
Chapter 5: Cytometer QC and Setup
49
Performing Automated Setup
50
Running Setup Using Manual Loading
51
Running Setup Using the Loader
61
Optimizing with BD Facscanto Clinical Software
64
Maintaining User-Specific Optimization Settings
70
Using Cytometer Controls
71
Reviewing Levey-Jennings Reports
74
Optimizing with BD Facsdiva Software
76
Verifying Cytometer Configuration and User Preferences
77
Creating the Experiment
78
Applying the Setup Results
81
Creating Compensation Controls
82
Optimizing Cytometer Settings
83
Calculating Compensation
87
Chapter 6: Running Samples with BD Facscanto Clinical Software
89
Running an Acquisition Worklist
90
Entering Information into a Worklist
91
Running a Process Control
92
Acquiring Samples
93
Options During Acquisition
99
Stopping Recording
106
Importing a Worklist from SPA Software
107
Reviewing an Analysis Worklist
108
Creating a New Analysis Worklist
108
Reviewing a Worklist
111
Logging out
113
Chapter 7: Running Samples with BD Facsdiva Software
115
Setting up the Global Worksheet
116
Recording Data
118
Importing a Worklist from SPA Software
120
Analyzing Data
122
Reusing the Analysis
125
Saving the Analysis
125
Logging out
127
Chapter 8: Using the Loader with BD Facsdiva Software
129
Getting Ready
130
Assigning Carousels and Verifying Run Settings
132
Preparing the Loader
136
Running Samples
139
Skipping or Re-Running Samples
141
Adding Tubes to an Existing Carousel
144
Re-Running a Carousel
144
Stopping a Run
144
Running a Single Tube
145
Running Cleaning Tubes on the Loader
146
Chapter 9: Shutting down
147
BD Facscanto Clinical Software
147
BD Facsdiva Software
149
Chapter 10: Maintenance
153
Scheduled Maintenance
154
Emptying the Waste Container
156
Purging the Fluidics Filters
158
Decontaminating the Fluidics System (Long Clean)
160
Replacing the Air Filter
161
Replacing Fluidics Filters
162
Unscheduled Maintenance
164
Changing a Cubitainer
166
Cleaning External Surfaces
171
Removing Bubbles from the Flow Cell
172
Cleaning the Flow Cell
173
Purging the Bubble Filter
174
Decontaminating the Fluidics System for Storage
175
Replacing the Bal Seal
175
Reconnecting the Fluidics Cart Tubing
181
Replacing the Fluidics Level Sensors
183
Replacing the Fluidics Cart Fuses
186
Chapter 11: Troubleshooting
189
Cytometer Troubleshooting
190
Loader Troubleshooting
195
Fluidics Cart Troubleshooting
198
BD Facscanto Clinical Software Troubleshooting
199
BD Facscanto Software General Issues
199
BD Facscanto Software Setup Wizard Messages
203
BD Facscanto Software Setup Report Failure Messages
205
BD Facscanto Software Levey-Jennings Errors and Messages
207
BD Facscanto Software Acquisition
207
BD Facscanto Software TBNK Analysis QC Messages
210
BD Facscanto Software Four- and Six-Color TBNK
212
BD Facsdiva Software Troubleshooting
215
BD Facsdiva Software General Issues
215
BD Facsdiva Software Cytometer Setup
219
BD Facsdiva Software Acquisition
220
BD Facsdiva Software Analysis
224
Display Troubleshooting
225
Appendix A: Supplies and Replacement Parts
227
Cytometer Supplies
228
Installation Kit
228
Other Replacement Parts
229
Barcode Reader Parts
229
Consumables
230
Cytometer Setup
230
Reagents
230
Labware
231
Appendix B: Technical Specifications
233
Cytometer Specifications
234
Environment
235
Performance
235
Optics
235
Fluidics
237
Signal Processing
237
Fluidics Cart Specifications
238
Capacity
238
BD FACS Loader Specifications
239
Index
241
Other manuals for BD FACSCanto II
Reference Manual
158 pages
User Guide
184 pages
Quick Reference Guide
6 pages
4
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BD FACSCanto II Specifications
General
Brand
BD
Model
FACSCanto II
Category
Measuring Instruments
Language
English
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