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General| Type | Flow Cytometer |
|---|---|
| Software | BD FACSDiva Software |
| Sorting Capability | No |
| Sample Volume | 12 x 75 mm or 12 x 17 mm tubes, 96-well plates |
| Sample Throughput/Data Acquisition Rate | Up to 10, 000 events/second |
| Applications | Immunophenotyping, cell cycle analysis, apoptosis |
| Sample Types | Cells, Beads, Particles |
| Weight | 136 kg (300 lb) |
| Laser Configuration | 3 lasers (488 nm blue, 633 nm red, 405 nm violet) |
Describes the system's components and their basic operational principles.
Illustrates and labels the main components of the flow cytometer.
Explains the components related to fluid handling and their operation.
Describes the optical components and how they detect light signals.
Lists the necessary software, workstation, and fluid requirements.
Explains the main window components of the BD FACSCanto clinical software.
Describes the standard and worklist toolbars for quick command access.
Outlines the main application windows and workspace layout in BD FACSDiva.
Provides instructions for connecting and operating the barcode reader.
Instructions for powering on the main cytometer unit.
Steps to launch and log into the cytometer control software.
Verifies the software's connection status with the cytometer.
Instructions for checking fluid levels and identifying low levels or full waste.
Procedure to check the flow cell for air bubbles and perform de-gassing.
Explains how to run automated setup and QC using setup beads.
Describes the steps for performing setup when loading samples manually.
Outlines the process for running setup with the automated sample loader.
Guides on adjusting settings like thresholds and voltages in the clinical software.
Details how to adjust FSC, SSC, and voltage settings for sample analysis.
Explains the process of calculating compensation for fluorescent signals.
Explains how to set up and run a group of samples using a worklist.
Details how to input sample information, IDs, and panel choices into a worklist.
Describes running a process control to validate optimized settings before sample analysis.
Guides the user through the process of acquiring data from samples.
Lists available actions that can be performed while acquiring data.
Guides on creating and configuring a global worksheet for data preview and recording.
Details the process of recording sample data using the software.
Explains how to analyze recorded sample data, including gating and statistics.
Outlines initial steps and requirements for using the loader.
Details how to configure carousels and check run settings for the loader.
Instructions for physically preparing the loader for sample acquisition.
Step-by-step guide for running samples using the automated loader.
Steps for shutting down the system using BD FACSCanto clinical software.
Steps for shutting down the system using BD FACSDiva software.
Lists routine maintenance tasks and their recommended frequency.
Describes maintenance procedures performed as needed to address specific issues.
Addresses common problems related to the cytometer's functionality.
Helps resolve problems encountered with the BD FACSCanto clinical software.
Assists in troubleshooting issues related to the BD FACSDiva software.
Troubleshooting when the cytometer does not respond to software.
Troubleshooting steps when the cytometer and cart do not power on.
Resolves issues when the aspirator arm is locked and prevents tube unloading.
Resolves issues when a test tube is stuck on the SIT and software is unresponsive.
Resolves issues when the fluidics cart does not power on.
Addresses general problems and error messages within the software.
Troubleshooting connection issues between software and cytometer.
Addresses issues with placing beads on scale or finding setup beads.
Addresses general problems and error messages within the software.
Troubleshooting when the software cannot connect to the cytometer.
Resolves issues when the cytometer shows a status message and is unresponsive.
Steps to resolve software unresponsiveness.
Further steps to resolve software unresponsiveness.
Troubleshooting compensation calculation errors.
Solutions for when data acquisition stops unexpectedly.
