212 BD FACSCanto II Instructions for Use
BD FACSCanto Software Four- and Six-Color TBNK
The following examples provide some assistance in troubleshooting BD Multitest
reagent plots.
Observation Possible Causes Recommended Solutions
Cell populations in
CD45 vs SSC extend
upward
Inadequate lysing of
sample
Prepare sample again, and
ensure complete lysis.
Granulocytes with low
side scatter in CD45 vs
SSC plot, no distinct
monocyte population
Aged blood or stained
cells
Refer to the reagent package
insert for stability limitations.
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