BD FACSCanto II - BD FACSCanto Software Acquisition; No events in plots after clicking Run, acquisition indicator lights are blinking as expected (viewing normal worksheet)

Chapter 11: Troubleshooting 207
BD FACSCanto Software Levey-Jennings Errors and
Messages
Observation Possible Causes Recommended Solutions
LJ plots empty, no data,
no error message appears
BD FACS Setup Beads-7
colors LJ.csv file missing
• If the file was renamed,
rename it with its original
name, and click Refresh.
• If the file was moved from the
default directory, move it
back, and then click Refresh.
LJ plots empty, no data,
error message appears
BD FACS Setup Beads-7
colors LJ.csv file
corrupted or contains
invalid data
Delete the BD FACS Setup
Beads-7 colors LJ.csv file, and
run setup again. The software
will create a new set of plots.
BD FACS Setup Beads-7
colors LJ.csv file open or
in use by another
application
1 Close the file or application.
2 Click Refresh.
BD FACSCanto Software Acquisition
Observation Possible Causes Recommended Solutions
No events in plots after
clicking Run,
acquisition indicator
lights are blinking as
expected
Threshold not set to
correct parameter
Set the threshold to the correct
parameter for your application.
Detector voltage too low Increase the voltage.
No events in plots after
clicking Run;
acquisition indicator
lights, except for those
corresponding to
threshold parameters,
are blinking as expected
Threshold too low or too
high
Adjust the threshold.
Threshold not set to
correct parameter
Set the threshold to the correct
parameter for your application.