BD FACSCanto II - BD FACSCanto Software Setup Wizard Messages; No acquisition events were received from cytometer; Failed to place [name of beads] on scale. or Failed to find [name of beads]; No valid data points

Chapter 11: Troubleshooting 203
BD FACSCanto Software Setup Wizard Messages
Messages Possible Causes Recommended Solutions
No acquisition events
were received from
cytometer
Communication problem
between hardware and
software
1 Turn on the power.
2 Connect the Ethernet cable to
the cytometer and computer.
3 Shut down the software,
computer, and cytometer, and
restart them.
4 To prevent carryover, perform
a fluidics startup before
acquiring data. Choose
Cytometer > Fluidics Startup.
Bubbles in flow cell Check for bubbles. If found, run
De-gas Flowcell.
Laser failure Check the laser power in the
Status window. If outside the
acceptable range, call
BD Biosciences.
Clogged SIT Clean the flow cell.
Failed to place [name of
beads] on scale.
or
Failed to find [name of
beads]
Bubbles in flow cell Check for bubbles. If found, run
De-gas Flowcell.
Software using saved
settings from a failed
setup
Delete the SetupResults.dat file
from C:\Program Files\
Common Files\BD\Setup
Results, and run setup again.
No valid data points Internal setup error Delete the SetupResults.dat file
from C:\Program Files\
Common Files\BD\Setup
Results, and run setup again.