116 BD FACSCanto II Instructions for Use
Setting Up the Global Worksheet
This section shows you how to use a global worksheet to preview and record
data for multiple samples. To switch between the normal and global worksheet
view, click the Global Worksheets button on the Worksheet toolbar ( ).
1 Open the experiment in which you optimized cytometer settings.
2 Create a new specimen ( ); rename it LW B.
3 Create two tubes under the LWB specimen, and rename them.
For example, TBNK_001 and TBNK_002.
To create a second tube, select the specimen, and click ( ).
4 Create a global worksheet; rename it Record Data.
•If the Default global worksheet preference is enabled in User
Preferences (default option), the global worksheet is already present.
Expand the Global Worksheets folder to locate and rename the
worksheet.
•If the Default global worksheet preference is disabled, create a global
worksheet by clicking the New Global Worksheet button in the
Browser toolbar ( ).
5 Use the Experiment Layout dialog to define parameter labels and to specify
the number of events to record for each tube.
Labels will appear on the plot axes and in all statistics views.
• Choose Experiment > Experiment Layout.
BD FACSDiva software v5.0 is required to run the BD FACSCanto II flow
cytometer. Older versions of BD FACSDiva software will not work with a
BD FACSCanto II flow cytometer.
To Next Page
Loading...
