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BD FACSCanto II - Loader Troubleshooting; Tube does not load; Tube does not unload

BD FACSCanto II
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Chapter 11: Troubleshooting 195
Loader Troubleshooting
Observation Possible Causes Recommended Solutions
Tube does not load Tube not completely raised Ensure the Loader drawer is
completely shut.
Ensure the tube is free of bulky
labels or tape.
Tube lifter hitting carousel
during ascent
Ensure the carousel is properly
engaged with the alignment guide
pin. If the problem persists,
contact BD Biosciences for
assistance.
Tube lifter failure Contact BD Biosciences.
Tube does not unload Tube lifter failure
1 If the tube lifter is in the up
position, turn off the cytometer
or Loader power for
approximately 5 minutes.
The air in the tube lifter will
decompress, lowering the tube
lifter.
2 Remove the tube.
If the problem persists, contact
BD Biosciences.
Status change not yet detected
by software
After replacing or emptying fluids,
wait until software detects a status
change before restarting
acquisition.
It can take up to 30 seconds for
the software to detect a fluid level
change.

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