196 BD FACSCanto II Instructions for Use
Tube missed, or missing
tube messages in
software
Salt crystal buildup on optical
sensors
Clean the tube optical sensor
(outer sensor), as described in
Cleaning External Surfaces on
page 171.
Condensation on tube Wipe tube.
Tube label reflection Turn tube.
Tube not detected Optical sensor is wet or dirty • Clean sensor
• Allow time for sensor to dry
Loader stalls or emits a
grinding noise
Door opened during Loader
initialization
When the Loader stops moving,
open and close the Loader doors.
Do not open the Loader doors
while the Loader is in motion.
Tube runs dry, Loader
not advancing to next
sample
Dilute sample or rare events Set a stopping time in
BD FACSDiva software.
See step 2 on page 139.
Bubble in flow cell diverts
stream
Degas flow cell. See Removing
Bubbles from the Flow Cell on
page 172.
Communication error
1 Restart the system.
2 Perform fluidics startup.
See step 5 on page 47.
Loader Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
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