Chapter 5: Cytometer QC and Setup 87
18 When acquisition stops, remove the tube.
Calculating Compensation
For these adjustments, you will need a single-stained control sample for each
parameter to be measured.
1 Install the first stained control tube onto the cytometer.
2 In the Acquisition Dashboard, click .
This moves the current tube pointer to the next tube in the Browser.
3 Click .
4 Verify the P1 gate encircles the population of interest, and click Record.
5 When recording is finished, click Remove Tube.
6 Install the next tube onto the SIT.
7 Repeat steps 2 through 6 until data for all stained control tubes has been
recorded.
Adjusting Gates
1 Make sure the current tube pointer is set next to the first stained control
(FITC Stained Control) tube.
2 Double-click the FITC Stained Control tube in the Browser to locate the
corresponding plots on the normal worksheet.
Do not change the PMT voltages after the first compensation tube has been
recorded. To calculate compensation, all tubes must be recorded with the
same PMT voltage settings. If you need to adjust the PMT voltage for a
subsequent compensation tube, delete the current compensation specimen,
recreate the specimen and run all the compensation tubes again.
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