86 BD FACSCanto II Instructions for Use
• Release the aspirator arm.
SIT cleaning occurs when the aspirator arm comes to center.
NOTICE To prevent backflow into the tube, follow the tube removal
sequence exactly. For more information, see Cytometer Troubleshooting on
page 190.
• When the Progress dialog finishes, you can load the next tube onto the
SIT.
12 Install the stained sample tube on the cytometer and click .
• Push the aspirator arm to the left.
• Place the tube onto the SIT, ensure the tube is straight, and firmly push
up until the tube comes to a complete stop and is fully seated.
• Center the aspirator arm under the tube. There are three sensor pins on
the aspirator arm. The bottom of the tube should sit within the center
of the pins.
13 If necessary, decrease the voltages to place the positive population for each
fluorescent parameter on scale.
14 Click to stop acquisition.
15 Remove the stained sample tube.
16 Re-install the unstained sample tube and click .
17 Wait 3 to 5 seconds, then click to record data for the
unstained control tube.
Data acquisition stops when acquisition criteria have been met.
To Next Page
Loading...
