Chapter 5: Cytometer QC and Setup 85
7 Select all fluorescence histograms on the Unstained Control worksheet.
8 In the Plot Inspector, select the Grid checkbox:
Gridlines are used to delineate log decades on plots. In a four-log display,
values are displayed from 26–262,143. Thus, the first log decade ranges
from 26–262. The lines are shown only in plots that display log parameters.
9 Optimize the voltages to place the negative population for each fluorescent
parameter on scale.
10 Click to stop acquisition.
11 When acquisition stops, remove the unstained control tube.
• Hold the sample tube while you push the aspirator arm to the left.
• Remove the tube from the SIT.
If you move the arm without holding the tube, the tube could fall off the
SIT and expose you to potentially biohazardous sample.
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