BD FACSCanto II - BD FACSDiva Software Acquisition; No events in plots after clicking Acquire, acquisition indicator lights are blinking as expected (viewing normal worksheet); No events in plots after clicking Acquire; (viewing normal or global worksheet)

220 BD FACSCanto II Instructions for Use
BD FACSDiva Software Acquisition
Observation Possible Causes Recommended Solutions
No events in plots after
clicking Acquire,
acquisition indicator
lights are blinking as
expected (viewing
normal worksheet)
(viewing normal or
global worksheet)
Current tube pointer not
on current tube
Move the current tube pointer in
front of the appropriate tube.
Viewing plots for a
different tube
Double-click the current tube in
the Browser to display the plots
for that tube.
Incorrect population(s)
in plot
Right-click the plot and choose
Show Populations; inspect the
populations.
Threshold not set to
correct parameter
Set the threshold to the correct
parameter for your application.
Detector voltage too low Increase the voltage.
No events in plots after
clicking Acquire;
acquisition indicator
lights, except for those
corresponding to
threshold parameters,
are blinking as expected
Threshold too low or too
high
Adjust the threshold.
Threshold not set to
correct parameter
Set the threshold to the correct
parameter for your application.