118 BD FACSCanto II Instructions for Use
Recording Data
If you want to save a copy of the data displayed in the global worksheet with
each recorded tube, enable the Save analysis after recording through global
worksheet preference in the User Preferences window (Figure 5-9 on page 78). If
you do not want to save a copy of the data, leave the preference disabled.
NOTICE If the software fails to respond during acquisition and you need to
restart the software, perform a fluidics startup prior to resuming acquisition. See
BD FACSDiva Software General Issues on page 215 for more information.
1 Install the first sample tube onto the cytometer.
• Push the aspirator arm to the left.
• Place the tube on the SIT, ensure the tube is straight, and firmly push
up until the tube comes to a complete stop and is fully seated.
• Center the aspirator arm under the tube. There are three sensor pins on
the aspirator arm. The bottom of the tube should sit within the center
of the pins.
2 In Acquisition Setup (Figure 7-2 on page 119), verify that you are using an
appropriate flow rate and that the SIT Flush checkbox is selected.
Tip Hide or show acquisition setup information by right-clicking the
dashboard anywhere outside the Basic Controls area and selecting the
option from the menu.
Always wear gloves when manually loading samples. A fluid flush of the
exterior of the sample injection tube (SIT) occurs between samples that
might contain biohazardous waste.
BD recommends leaving the SIT Flush option enabled to minimize
carryover between tubes.
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