Chapter 11: Troubleshooting 205
There is a float switch
error
Sheath tank not attached
to fluidics cart
Reconnect sheath cubitainer or
tubing, and remove kinks.
Sheath tubing from
fluidics cart to cytometer
not attached or pinched
Sheath filter not primed Always prime after changing a
cubitainer.
Fluidics airlock See Removing an Air Lock on
page 169.
Fluidics cart off or
disconnected
Switch on the fluidics cart circuit
breaker; make sure the power
cable is connected at both ends.
Low or no pressure • Check air supply connections.
• Check air pressure gauge.
BD FACSCanto Software Setup Report Failure
Messages
Failed Possible Causes Recommended Solutions
Detector voltage Bubbles in flow cell Check for bubbles. If found, run
De-gas Flowcell.
Wrong target value used Match the target values in the
software to those printed on the
BD FACS 7-color setup beads label.
Re-enter them, if needed.
Setup.dat file deleted Accept the failed setup, and then re-
run Setup.
BD FACSCanto Software Setup Wizard Messages
Messages Possible Causes Recommended Solutions
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