BD FACSCanto II - Page 206

206 BD FACSCanto II Instructions for Use
Sensitivity Bubbles in flow cell Check for bubbles. If found, run
De-gas Flowcell.
Dirty flow cell Clean the flow cell.
Spectral overlap Expired bead lot Run setup again with fresh tube of
setup beads.
Software using saved
setting from a failed
setup
Delete the SetupResults.dat file from
C:\Program Files\Common
Files\BD\Setup Results, and run
setup again.
Incorrect spectral
overlap factors used
Match the spectral overlap factors
in the software to those printed on
the BD FACS 7-color setup beads
label.
Laser power Laser failure Check the laser power in the Status
window. If outside the acceptable
range, call BD Biosciences.
Sheath pressure Kinked or clogged sheath
line
Remove any kinks in tubing to the
fluidics cart.
Clogged or airlocked
sheath filter
Check the sheath filter. Open the
bleeder valves. If no fluid leaks out,
remove the air lock.
BD FACSCanto Software Setup Report Failure
Messages (continued)
Failed Possible Causes Recommended Solutions