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Explains conventions used in the instructions, including hazard symbols and text formatting.
Overview of the BD FACSCalibur system, including the flow cytometer and workstation.
Details of the BD FACSCalibur sensor unit and its components.
Explanation of how samples are carried to the flow cell and the role of the fluidics system.
Explanation of the BD FACSCalibur optics system, including lasers and detectors.
Diagram and explanation of the optics system when the FL4 option is installed.
Description of the BD FACS Loader option for automated acquisition from tubes.
Details about the carousel rack's capacity and features for holding sample tubes.
Explanation of the keypad used to operate the BD FACS Loader manually.
Information on how to operate the main power switches for the cytometer and transformer.
Hardware and software requirements for operating the BD FACSCalibur system.
Software for installing required startup files or BD Inits for acquisition.
Software for general data acquisition and analysis.
Software for instrument calibration and quality control.
Configuration options for BD FACSComp software, including optimization and export.
Description of calibration files, target files, and summary reports generated by FACSComp.
Software for identifying lymphocytes and subsets in stained samples.
Tools within BD Multiset for defining ranges, creating panels, and managing comments.
Types of files created by BD Multiset software, including schedule, reports, and data files.
Software for determining HLA-B27 antigen expression.
Configuration options for BD HLA-B27 software, including export, printing, and scaling.
Types of files created by BD HLA-B27 software, including schedule, reports, and data files.
Description of the BD FACS Loader software and its integration.
Settings for Worklist Manager, including loader communication and custom columns.
File types created by Worklist Manager, such as worklist documents and summary reports.
Procedure for filling the sheath reservoir to 75% capacity.
Procedure to remove bubbles from the flow cell using the PRIME function.
Procedure for emptying the waste reservoir to prevent overflow.
Steps for launching BD FACSComp software and selecting assays for quality control.
Instructions for running BD FACSComp software for instrument QC.
Procedure for manually acquiring data for instrument QC.
Adjusting instrument settings to optimize performance and ensure accurate results.
Methods for maintaining a log of instrument quality control results.
Steps to create or adapt a Standard Acquisition Experiment document for sample analysis.
Procedure to adjust scatter detectors and threshold for optimal sample display.
Creating polygon regions to define and isolate cell populations in scatter plots.
Adjusting detector voltages to remove background fluorescence and optimize settings.
Adjusting settings to correct for spectral overlap between fluorophores.
Saving instrument settings to a file for later restoration.
Steps for acquiring sample data, including specifying file name and location.
Defining the number of events to acquire and store, and specifying data storage.
Associating reagent names with fluorescence parameters for clearer data analysis.
Creating panels to group reagent combinations for multiple experiments or donors.
Associating plots with tubes in the Browser using defined panels.
Associating custom instrument or acquisition settings with specific tubes in a panel.
Procedures for saving acquired data for each tube.
Steps for analyzing acquired FCS data files using BD CellQuest Pro software.
Steps to create a dot plot and display data from a selected file.
Generating a contour plot showing gated data from a selected file.
Selecting and editing quadrant statistics to display in analysis reports.
Using batch analysis to analyze multiple data files and export statistics.
Creating application-specific documents with defined acquisition conditions for Worklist Manager.
Launching and signing into BD Worklist Manager software.
Associating instrument settings with panels and customizing mix settings.
Instructions for running BD FACSComp software with the Loader.
Saving worklist information to use as a template for daily worklists.
Entering sample details manually, via barcode, or by importing a worklist.
Assigning tube positions to samples and defining rack locations.
Loading racks onto the Loader and initiating sample acquisition.
Procedures for pausing or stopping the Loader during acquisition.
Performing full-panel reruns of finished or stopped worklists.
Procedures for daily shutdown, including optional cleaning after automatic clean.
Routine maintenance procedures performed on a regular schedule.
Procedure for cleaning the system fluidics monthly or more frequently.
Instructions for replacing the sheath filter to trap debris.
Maintenance tasks performed as needed, not on a fixed schedule.
Procedure for replacing the Bal seal to ensure proper tube pressurization.
Procedure for replacing the sample O-ring for droplet containment vacuum.
Procedures for maintaining the BD FACS Loader.
Common issues and solutions related to the instrument's hardware and basic functions.
Solutions for problems encountered during sample acquisition.
Troubleshooting common issues with the BD FACSComp software.
Troubleshooting common error codes and issues with BD Multiset software.
Solutions for problems encountered with the BD FACS Loader.
List of general instrument supplies and their reorder numbers.
List of items included with the instrument, with reorder numbers.
List of replacement parts for the instrument, with part numbers.
List of consumable items used with the instrument, such as tubes and pads.
Catalog numbers and purposes for BD Calibrite beads and kits.
List of reagents used with the instrument, including catalog numbers.
Catalog numbers for labware items like test tubes and absorbent pads.
Physical dimensions, weight, power requirements, and consumption of the cytometer.
Operating temperature, humidity, noise level, and facility requirements.
Fluorescence and scatter sensitivity, resolution, and carryover specifications.
Specifications for lasers, excitation optics, and emission optics.
Details on general operation, fluid reservoirs, pressure, and flow rates.
Workstation resolution, data acquisition channels, and fluorescence compensation.
Compatibility and specifications for the BD FACS Loader, including racks and tubes.
Schematics showing connections between the cytometer, computer, and Loader.
Regression analysis comparing BD Multitest CD3/CD8/CD45/CD4 results.
Within-specimen reproducibility studies for subset percentages and absolute counts.
Evaluation of linear range for WBC and lymphocyte concentrations.
General| Type | Flow Cytometer |
|---|---|
| Detectors | Photomultiplier tubes (PMTs) |
| Data Acquisition Rate | Up to 10, 000 events per second |
| Software | BD CellQuest Pro |
| Laser Configuration | 488 nm blue laser, 635 nm red diode laser |
| Fluorescence Channels | 4 |
| Sample Input | Test tubes or microcentrifuge tubes |
| Sample Flow Rate | Adjustable |
| Data Acquisition | BD CellQuest™ software |
| Analysis Software | BD CellQuest Pro |
| Sample Tube Capacity | 12 x 75 mm tubes |
| Dimensions | 76 cm x 61 cm |
