Chapter 9: Troubleshooting 195
BD FACSComp Troubleshooting
PMT Adjustment
Observation Possible Causes Recommended Solutions
Unable to set PMT
voltages automatically
Event rate below 400 • Mix tube.
• Set the flow rate to HI.
• Add beads to tube for which
event rate appears low.
75-second time limit
surpassed
Adjust settings manually for
PMTs without checkmarks
(page 71, step 4).
Air bubble in flow cell Prime the fluidics (page 58).
Dirty flow cell or partial
clog
Perform monthly cleaning
(page 171).
>100-volt difference in
PMT voltages
Contaminated beads Prepare new beads.
Air bubble in flow cell Prime the fluidics (page 58).
Dirty flow cell or partial
clog
Perform monthly cleaning
(page 171).
Incorrect APC bead
lot ID
Enter the correct APC bead lot
ID.
New target values for
beads
Verify new target values are
correct.
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