170 BD FACSCalibur Instructions for Use
Scheduled Maintenance
Maintenance
Procedure
Description and Location Frequency
Filling the sheath page 56 Daily and as needed
Emptying the waste page 58 Daily and as needed
Daily shutdown page 165 Daily and immediately after
running viscous samples or
dyes such as propidium
iodide (PI), acridine orange,
or thiazole orange.
Cleaning the fluidics Decontaminates the sheath
tubing, waste tubing, and flow
cell with cleaning solution, then
rinses with DI water.
See page 171.
Monthly and before service
calls
Replacing the sheath
filter
Keeps fluids free of particulates
See page 173.
Every 3–6 months or when
increased debris seen in
FSC vs SSC plots
Scheduling preventive
maintenance by a
qualified service
engineer
— Every 6 months
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