Chapter 9: Troubleshooting 201
General BD Multiset Software
Observation Possible Cause Recommended Solution
Disappearing dots on
printouts
Wrong settings in Print
Setup
Choose grayscale or color in
Print setup, not black and
white.
Differing CD3 counts
within panel
Pipetting error Use fully charged, calibrated
electronic pipette (or
equivalent) that delivers a
precise amount of sample.
Make sure sample does not
drip from pipette tip during
sample preparation.
Tube run out of order,
wrong reagent used, or
different samples in same
panel
Take care to run tubes in
order. Do not mix samples in
the same panel.
Cell and/or BD Trucount
bead settling
• Vortex tubes before
acquiring. Resuspend cells
if tubes have been sitting
too long.
• Adjust mix settings.
• Restain sample, if
necessary.
Absolute count bead
attractor missing bead
population
Adjust bead attractor at
Manual Gate view.
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