190 BD FACSCalibur Instructions for Use
Instrument Troubleshooting
Observation Possible Causes Recommended Solutions
Droplet containment
vacuum not
functioning
Worn O-ring in retainer Replace the O-ring (page 178).
Outer sleeve missing or
not seated in retainer
1 Loosen the retainer.
2 Push up the outer sleeve until fully
seated.
3 Tighten the retainer.
Waste line pinched,
insufficient aspiration
Check the waste line.
Flow cell not filling No sheath pressure • Verify the vent valve is forward.
• Tighten the sheath cap.
• Check that all sheath connectors are
securely attached.
• Check for leaks or cracks in the sheath
reservoir. Replace it, if needed.
• Check the Status sample voltage, in
RUN with test tube off (page 112).
Empty sheath reservoir Fill the sheath reservoir.
Air in sheath filter Vent air from the sheath filter. See
page 57, step 14.
FL4–%FL3
compensation more
than twice usual
Loose sheath cap Tighten the sheath cap.
Air in sheath filter Vent air from the sheath filter. See
page 57, step 14.
Air bubble in flow cell Prime the fluidics (page 58).
Inaccurate timing
between blue and red
laser
Perform Time-Delay Calibration
(page 72).
To Next Page
Loading...
