BD FACSCalibur - BD Multiset Results

Chapter 9: Troubleshooting 203
BD Multiset Results
Observation Possible Cause Recommended Solutions
Cell populations outside
attractors
Donor-specific anomaly • Manually adjust
attractor and save as
user-defined reagent
(page 134).
• If all samples show
same anomaly, rerun
BD FACSComp
software and optimize
settings for application
(all but compensation).
Rerun sample.
CD3 FITC
SSC-H
SSC-H
CD45 PerCP
CD3 FITC
CD19 PE