220 BD FACSCalibur Instructions for Use
Optics
Laser Specifications
The following Class 3B lasers are mounted on the BD FACSCalibur instrument.
Because the lasers are contained within the instrument, the BD FACSCalibur is a
Class 1 (I) laser product.
Excitation Optics
Emission Optics
Manufacturer Model Wavelength (nm) Power (mW)
JDS Uniphase® 2111D-015SLBD 488 15
(optional second laser) Coherent® HL6322G (Hitachi) 635 10
Optical platform Fixed optical assembly
Beam geometry Blue laser: 22 µm x 66 µm elliptical beam
Red laser: 15 µm x 61 µm elliptical beam
Collection lens Optical gel–coupled to quartz cuvette
Fluorescence detection 4 PMT detectors (standard) + 1 optional
3 wavelengths detected from 488-nm laser:
• 530/30 nm
• 585/42 nm
• >670 nm
One wavelength detected from optional 633-nm
laser: 661/16 nm
Forward scatter detection Photodiode with 488/10 bandpass filter
Side scatter detection PMT with Brewster-angle beam splitter
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