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7
Shutting Down
To ensure consistent instrument functioning, always clean the instrument before
you turn it off at the end of the day.
Do the following before instrument shutdown and immediately after running
viscous samples or dyes such as propidium iodide (PI), acridine orange, or
thiazole orange.
; Tip If you will not be using your instrument for a week or longer, decontaminate
the fluidics (page 171) and keep DI water in the fluidics system until you use the
instrument again.
1 Install a tube containing 3 mL of cleaning solution.
For cleaning, use BD FACS Clean, a 1:10 dilution of bleach in DI water, or
0.5% sodium hypochlorite.
2 Move the support arm to the side and allow the vacuum to aspirate 2 mL
of the solution.
3 Move the support arm to the center and run the cleaning solution on HI for
5 minutes.
4 Install a tube containing 3 mL distilled (DI) water.
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