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BD FACSCalibur - Changing the Sheath Filter

BD FACSCalibur
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Chapter 8: Maintenance 173
Changing the Sheath Filter
The sheath filter traps debris before it reaches the flow cell. Replace the filter
every 3–6 months or when you see increased debris in an FSC vs SSC plot.
1 Open the fluidics drawer.
2 Push the vent valve toward the arrow to depressurize the system.
3 Disconnect the output and input quick-disconnects.
4 Unscrew the air vent tubing fitting from the filter vent port.
Ensure the filter is free of the instrument and the air vent tubing is attached
to the instrument.
5 Unscrew and remove the base from the filter body.
Save this piece to attach to the new filter.
6 Pull the output tubing from the output port.
output quick-disconnect
filter
input quick-disconnect
vent port
O-ring
base

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