160 BD FACSCalibur Instructions for Use
Running Samples
1 Gently vortex each sample tube and place it in the rack according to the
Rack Manifest.
If cleaning tubes are required, install a tube containing 3 mL of 10% bleach
in position 39 and a tube containing 3 mL of DI water in position 40.
NOTICE If loaded racks sit a long time before the samples are run, vortex
the tubes again before proceeding.
2 Install the rack on the Loader.
Position the spindle hole over the center spindle of the Loader drawer,
rotate the rack until the alignment guide pin fits into the small hole on top
of the rack, and press down firmly.
Figure 6-2 Installing the Loader rack
3 Close the Loader drawer completely, and install the Loader cover.
The Loader scans and positions the rack at tube Position 1.
For accurate results, make sure the tube loading sequence matches what is
printed on the Rack Manifest.
To operate the Loader, the cover must be in place on the Loader drawer.
Tubes will not be loaded if the cover is off, and currently running tubes will
be unloaded if the cover is removed during a run.
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spindle hole
alignment hole
rack handle
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