BD FACSCalibur User Manual

Chapter 4: Instrument QC 77
• Side Scatter Sensitivity Test
The cytometer produces an electronic test pulse in the FSC parameter.
BD FACSComp software acquires 10,000 events, locates the upper test
pulse peak channel, then acquires data for the side scatter Sensitivity Test.
The separation between noise and the side scatter signal of the beads is
calculated. When complete, test pulses are automatically turned off.
• Forward Scatter Sensitivity Test
The software searches for the upper level of FSC noise by lowering the FSC
threshold. After it finds the noise level, the software sets the threshold,
acquires 10,000 events, and calculates the separation between the threshold
and the FSC mean channel of the beads.
Viewing Results
After the Sensitivity Test, a Summary Report is displayed in a scrollable window.

BD FACSCalibur Specifications

General

Brand	BD
Model	FACSCalibur
Category	Measuring Instruments
Language	English