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BD FACSCalibur - Page 78

BD FACSCalibur
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78 BD FACSCalibur Instructions for Use
The Summary Report shows histograms and the separations for each parameter,
current instrument settings, time-delay calibration results for 4-color setup, and
Sensitivity Test results. Two files are saved if specified at the Set Up view: a text
file (without graphics) and a PDF file (image of the report). Both files can be
opened and printed using standard Macintosh applications.
1 Remove the mixed-bead tube and press STNDBY.
2 Review the report and enter comments, if needed.
If any parameter failed, see page 199 for troubleshooting.
To enter comments, scroll to the Comments section, click to insert the
cursor, and type. If you exceed three lines, text flows to a new page.
NOTICE Comments can be entered only before the file is saved. You
cannot add comments after you leave the Summary view.
3 Choose File > Print, if needed.
If you selected automatic printing in print preferences, the report will print
when you leave the view.
4 Select from the following.
Click Next Assay to proceed with the next assay.
If this was the last assay, click Set Up to return to the Set Up view.
Click Optimization (enabled after the last assay) to optimize settings.
See page 79.
Click Quit to quit the software.

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