144 BD FACSCalibur Instructions for Use
Acquiring Data
At the Acquisition view, sample data is shown in plots before and during
acquisition. Data is not saved until you click Acquire. Once a sample has been
run on the cytometer, sample information in the Samples view cannot be edited.
1 On the cytometer, press RUN and HI.
2 Vortex and install the first sample tube on the SIP.
Make sure the run order corresponds with the Samples view. For
acquisition with the Loader, see Chapter 6.
3 Click Run Tests at the Samples view.
BD HLA-B27 instrument settings are automatically sent to the cytometer.
The Acquisition view appears and events appear in the plots.
4 Click Acquire.
Acquisition proceeds until the designated number of events is acquired.