130 BD FACSCalibur Instructions for Use
4 Choose Cytometer > Instrument Settings to download instrument settings.
BD Multiset software, run as a standalone application, does not
automatically download instrument settings. You can manually download
settings at the Samples, Lab Report, Phys Report, and Summary views.
If you run samples requiring different optimized settings, download settings
at the Phys Report view between panels.
• Navigate to the BD Files > Instrument Settings Files and double-click
TriTEST.opt or MultiTEST.opt. (You can also use Calib file.LNW or
any saved instrument settings file.)
• Click Set, and then Done.
5 Choose File > Save to save the Schedule document.
Acquiring Data
At the Acquisition view, sample data is shown in plots before and during
acquisition. Data is not saved until you click Acquire. Once a sample has been
run on the cytometer, sample information in the Samples view cannot be edited.
1 Vortex and install the first control tube on the SIP.
2 Press RUN and HI.
3 Click Run Tests at the Samples view.
Events appear in plots (Figure 5-6).
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