Appendix B: Technical Specifications 221
Fluidics
General operation Front panel control with three modes: RUN,
STNDBY, and PRIME; standby mode for conserving
sheath fluid when no sample tube installed
Fluid reservoirs 4-L sheath and waste containers in a pull-out
drawer; level detectors for automatic indicating of
low levels of sheath or high levels of waste
Sheath pressure 4.5 psi
Sample flow rates Regulated and monitored pressure difference
between sheath and sample; particle velocity in flow
cell approximately 6 meters/second
Low = 12 µL/min
Medium = 35 µL/min
High = 60 µL/min
Sample acquisition rate 2,000 events/sec
Recommended sample
concentration range
Single-cell suspension of 1 x 10
5
to 2 x 10
7
particles/mL
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